<a href='https://brc.riken.jp/mus/pcr01498'>Genotyping protocol -PCR-</a> 独立行政法人理化学研究所 Developed by Drs. Takaoki Kasahara and Tadafumi Kato at RIKEN BSI in 2001. The mutant POLG coding fragment including the CaMKII alpha promoter was injected into the pronuclei of C57BL/6J fertilized eggs. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Molecular Psychiatry, 11, 577-593 (2006).The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.<br>The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE when the RECIPIENT ordered the BIOLOGICAL RESOURCE before the publication of the paper indicated above. Cell Biology Research 通常の研究目的では、オス TG マウスを C57B6/J 野生型メスと繁殖させる。（メスより変異ミトコンドリア DNA が伝達されるのを防ぐため） 繁殖効率：A 行動の異常がある true Tadafumi KATO Bipolar Disorder Model Mouse Bipolar Disorder Model Mouse Bipolar disorder, a mood disorder marked by alternating periods of manic and depressive episodes, affects approximately 1% of the global population. Mitochondrial dysfunction has been seen in patients with bipolar disorder. Moreover, patients with mitochondrial disorders sometimes display mood disorders. This transgenic mice express mouse mitochondrial DNA polymerase (POLG) having a point mutation D181A under the control of CaMKII alpha promoter. The mutant mice exhibited characteristic behavioral phenotypes in accordance with criteria for the validation as an animal model of mood disorder. This strain is useful to investigate the cause of bipolar disorder and to develop new mood stabilizers to treat bipolar disorder. RIKEN C57BL/6J-Tg(Camk2-mutPolg)1Bsi C57BL/6J-Tg(Camk2-mutPolg)1Bsi Backcross to C57BL/6 (hemizygote male x 57BL/6JJcl female) Don't use Tg female for breeding in order to prevent transmission of mutated mtDNA. 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Molecular Psychiatry, 11, 577-593 (2006).<br>The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.<br>The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE when the RECIPIENT ordered the BIOLOGICAL RESOURCE before the publication of the paper indicated above. Backcross to C57BL/6 (hemizygote male x 57BL/6JJcl female)Don't use Tg female for breeding in order to prevent transmission of mutated mtDNA. mouse CaMKIIα promoter , mouse Polg (mitochondrial DNA polymerase γ) mutant cDNA, SV40 polyA 間発年：2001年開発者：笠原和起先生、加藤忠史先生機関名：理化学研究所　脳科学総合研究センター <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/may_2006_mm" target="_blank">Mouse of the Month May 2006</A> Mouse Models for Human Disease C（3〜6か月） 通常の研究目的では、オス TG マウスを C57B6/J 野生型メスと繁殖させる。（メスより変異ミトコンドリア DNA が伝達されるのを防ぐため）繁殖効率：A行動の異常がある 加藤　忠史 C (3-6 months) ミトコンドリア DNA 合成酵素の校正活性を失わせた変異体の遺伝子に、前脳特異的なプロモーター (CAMKIIα) を結合したトランスジーンを C57BL6/J に導入したトランスジェニック(TG) マウス。躁うつ病類似の行動異常を示すことから、気分安定薬の開発研究および躁うつ病の病態研究に有用と考えられる。 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.riken.jp/en/research/labs/cbs/res_resour/index.html" target="_blank">Lab HP</A> 間発年：2001年 開発者：笠原和起先生、加藤忠史先生 機関名：理化学研究所　脳科学総合研究センター RBRC01498